Loss of heterozygosity in mammalian cell mutagenesis: molecular analysis of spontaneous mutations at the aprt locus in CHO cells.

doi:10.1093/carcin/11.9.1485

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Article

Loss of heterozygosity in mammalian cell mutagenesis: molecular analysis of spontaneous mutations at the aprt locus in CHO cells.

Carcinogenesis - 1 Sept 1990

Ward M A, Yu M, Glickman B W, Grosovsky A J

Abstract excerpt

Loss of heterozygosity at previously heterozygous loci may occur by one of several possible mechanisms and account for a large fraction of all mutations occurring at such loci. In order to investigate loss of heterozygosity events, we have chosen the aprt locus of Chinese hamster ovary (CHO) cells as our model since it is readily available in either heterozygous or hemizygous form. Cloning and sequencing of the...

Topics

Adenine Phosphoribosyltransferase
Alleles
Animals
Base Sequence
Cell Line
Cloning, Molecular
Cricetinae
Cricetulus
Female
Heterozygote
Molecular Sequence Data

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Mutation

Oligonucleotide Probes

Ovary

Pentosyltransferases

Polymerase Chain Reaction

Polymorphism, Restriction Fragment Length

Source

Open in PubMed

Identifiers

PMID: 1976045
DOI: 10.1093/carcin/11.9.1485

Genes

APRT

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1976045-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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Loss of heterozygosity in mammalian cell mutagenesis: molecular analysis of spontaneous mutations at the aprt locus in CHO cells.PMID 1976045Read ↗

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