Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Loss of alleles in aprt mutants of CHO cells demonstrated by BclI restriction-fragment-length variation.

Somatic cell and molecular genetics - 1 May 1990

Dewyse P, Bradley W E

Abstract excerpt

A BclI restriction-fragment-length variation (RFLV) has been identified 7 kb downstream of the 3' end of the adenine phosphoribosyltransferase (aprt) gene in the Chinese hamster CHO cell line. This RFLV generates 19 kb and 17 kb + 2 kb BclI fragments. Three other cell lines derived from different Chinese hamsters are homozygous for this RFLV. The 17 kb + 2 kb allele was mapped to the Z4 chromosome based upon the...

Topics

Adenine Phosphoribosyltransferase
Alleles
Animals
Blotting, Southern
Cell Line
Chromosome Mapping
Chromosomes
Cricetinae
Deoxyribonucleases, Type II Site-Specific
Heterozygote
Homozygote

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Mutation

Pentosyltransferases

Polymorphism, Restriction Fragment Length

Restriction Mapping

Source

Identifiers

PMID: 1972816
DOI: 10.1007/BF01233358

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1972816-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

0

discussions

0

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Loss of alleles in aprt mutants of CHO cells demonstrated by BclI restriction-fragment-length variation.PMID 1972816Read ↗

Analysis of second-step mutations of class II and class III CHO aprt heterozygotes: chromosomal differences in deletion frequencies.Semantic proximity Read ↗

Loss of heterozygosity in mammalian cell mutagenesis: molecular analysis of spontaneous mutations at the aprt locus in CHO cells.Semantic proximity Read ↗

Use of gene targeting to study recombination in mammalian cell DNA repair mutants.Semantic proximity Read ↗

Characterization of an apparent hotspot for spontaneous mutation in exon 5 of the Chinese hamster APRT gene.Semantic proximity Read ↗

Reduction to homozygosity is the predominant spontaneous mutational event in cultured human lymphoblastoid cells.Semantic proximity Read ↗

Isolation of an APRT heterozygote from TK6 human lymphoblasts: predominance of multi-locus loss of heterozygosity among spontaneous APRT-mutants.Semantic proximity Read ↗

Use of gene targeting to study recombination in mammalian cell DNA repair mutants.Semantic proximity Read ↗

Complex chromosomal mechanisms lead to APRT loss of heterozygosity in heteroploid cells.Semantic proximity Read ↗

Loss of heterozygosity: the most frequent cause of recessive phenotype expression at the heterozygous human adenine phosphoribosyltransferase locus.Semantic proximity Read ↗

A mutational hotspot in the aprt gene of Chinese hamster cells.Semantic proximity Read ↗

Analysis of the promoter region of the CHO APRT gene.Semantic proximity Read ↗

Determination of spontaneous loss of heterozygosity mutations in Aprt heterozygous mice.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.