Article
Isolation of an APRT heterozygote from TK6 human lymphoblasts: predominance of multi-locus loss of heterozygosity among spontaneous APRT-mutants.
Mutation research - 9 Jun 1997
Pongsaensook P, Smith L E, Grosovsky A J
Abstract excerpt
The TK6 human B lymphoblastoid cell line contains two easily and widely used selectable markers: the X-linked, hemizygous hprt locus, and the heterozygous tk locus on chromosome 17q. In this study, rare APRT heterozygotes were directly isolated from the TK6 population by clonal selection in cell...
Topics
- Adenine
- Adenine Phosphoribosyltransferase
- B-Lymphocytes
- Clone Cells
- Deoxyribonucleases, Type II Site-Specific
- Drug Resistance
- Electrophoresis, Polyacrylamide Gel
- Heterozygote
- Humans
- Microsatellite Repeats
- Mutation
- Polymerase Chain Reaction
- Polymorphism, Genetic
- Polymorphism, Restriction Fragment Length
- Sequence Analysis, DNA
- Tumor Cells, Cultured