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Article

Introduction of a subtle mutation into the Hox-2.6 locus in embryonic stem cells.

Nature - 21 Mar 1991

Hasty P, Ramírez-Solis R, Krumlauf R, Bradley A

Abstract excerpt

Gene targeting in embryonic stem (ES) cells is a powerful tool for generating mice with null alleles. Current methods of gene inactivation in ES cells introduce a neomycin gene (neo) cassette both as a mutagen and a selection marker for transfected cells. Although null alleles are valuable, changes at the nucleotide level of a gene are very important for functional analysis. One gene family in which subtle...

Topics

Alleles
Animals
Base Sequence
Chromosome Mapping
Electrophoresis, Polyacrylamide Gel
Embryo, Mammalian
Enhancer Elements, Genetic
Genes, Homeobox
Hypoxanthine Phosphoribosyltransferase
Mice
Molecular Sequence Data

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Mutagenesis, Site-Directed

Promoter Regions, Genetic

Recombination, Genetic

Stem Cells

Transcription, Genetic

Source

Identifiers

PMID: 1672446
DOI: 10.1038/350243a0

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1672446-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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