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Article

Introduction of a subtle mutation into the Hox-2.6 locus in embryonic stem cells.

Nature - 21 Mar 1991

Hasty P, Ramírez-Solis R, Krumlauf R, Bradley A

Abstract excerpt

Gene targeting in embryonic stem (ES) cells is a powerful tool for generating mice with null alleles. Current methods of gene inactivation in ES cells introduce a neomycin gene (neo) cassette both as a mutagen and a selection marker for transfected cells. Although null alleles are valuable, changes at the nucleotide level of a gene are very important for functional analysis. One gene family in which subtle...

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PMID: 1672446
DOI: 10.1038/350243a0

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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