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Article

A simple two-step, 'hit and fix' method to generate subtle mutations in BACs using short denatured PCR fragments.

Nucleic acids research - 1 Aug 2003

Yang Yongping, Sharan Shyam K

Abstract excerpt

The bacteriophage lambda recombination system has proven to be a valuable tool for engineering bacterial artificial chromosomes (BAC). Due to its high efficiency, subtle alterations in the BACs can be generated using oligonucleotides as targeting vectors. Since no selection marker is used, recombinant clones are identified utilizing a selective PCR screening method. However, occasionally the selective PCR...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Identifiers

PMID: 12888532
DOI: 10.1093/nar/gng080
PMCID: PMC169966

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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