Article
High-throughput site-directed mutagenesis in ES cells.
Biochemical and biophysical research communications - 30 Nov 2001
Höllrigl A, Hergovich A, Görzer I, Bader A, Ellersdorfer G, Habegger K, Hammer E, Enzinger S, Capetanaki Y, Weitzer G
Abstract excerpt
Introduction of nonselectable mutations into the genome of embryonic stem cells by homologous recombination allows to investigate the function of genes at the molecular level and has been achieved, however, at very low efficiencies by the Hit and Run, Tag and Exchange, and Double Replacement strategies. Comparing those strategies at a single locus with vectors derived from a single fragment of the desmin gene led...
Topics
- Alleles
- Amino Acid Sequence
- Animals
- Antiviral Agents
- Cells, Cultured
- CpG Islands
- DNA, Complementary
- Desmin
- Embryo, Mammalian
- Ganciclovir
- Genetic Techniques