Article
Isolation of homozygous mutant mouse embryonic stem cells using a dual selection system.
Nucleic acids research - 1 Feb 2012
Huang Yue, Pettitt Stephen J, Guo Ge, Liu Guang, Li Meng Amy, Yang Fengtang, Bradley Allan
Abstract excerpt
Obtaining random homozygous mutants in mammalian cells for forward genetic studies has always been problematic due to the diploid genome. With one mutation per cell, only one allele of an autosomal gene can be disrupted, and the resulting heterozygous mutant is unlikely to display a phenotype. In cells with a genetic background deficient for the Bloom's syndrome helicase, such heterozygous mutants segregate...
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