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Article

An ES cell system for rapid, spatial and temporal analysis of gene function in vitro and in vivo.

Nucleic acids research - 12 Oct 2005

Mao Junhao, Barrow Jeffery, McMahon Jill, Vaughan Joe, McMahon Andrew P

Abstract excerpt

We describe a versatile genetic system for rapid analysis of mammalian gene function. In this, loss of reporter activity in a novel embryonic stem (ES) cell line enables rapid identification of targeting to the ubiquitously expressed Rosa26 locus. Subsequent regulation of gene activity is governed by a dual regulatory strategy utilizing two drugs, Tamoxifen and Doxycycline. To illustrate this approach, a dominant...

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Identifiers

PMID: 16221970
DOI: 10.1093/nar/gni146
PMCID: PMC1253836

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Source receipt: pubmed, retrieved 7 Sept 2026.

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