Article
Chemical rescue, multiple ionizable groups, and general acid-base catalysis in the HDV genomic ribozyme.
RNA (New York, N.Y.) - 1 Jul 2006
Perrotta Anne T, Wadkins Timothy S, Been Michael D
Abstract excerpt
In the ribozyme from the hepatitis delta virus (HDV) genomic strand RNA, a cytosine side chain is proposed to facilitate proton transfer in the transition state of the reaction and, thus, act as a general acid-base catalyst. Mutation of this active-site cytosine (C75) reduced RNA cleavage rates by as much as one million-fold, but addition of exogenous cytosine and certain nucleobase or imidazole analogs can...
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