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Article

Direct measurement of a pK(a) near neutrality for the catalytic cytosine in the genomic HDV ribozyme using Raman crystallography.

Journal of the American Chemical Society - 31 Oct 2007

Gong Bo, Chen Jui-Hui, Chase Elaine, Chadalavada Durga M, Yajima Rieko, Golden Barbara L, Bevilacqua Philip C, Carey Paul R

Abstract excerpt

The hepatitis delta virus (HDV) ribozyme uses a cytosine to facilitate general acid-base catalysis. Biochemical studies suggest that C75 has a pKa perturbed to near neutrality. To measure this pKa directly, Raman spectra were recorded on single ribozyme crystals using a Raman microscope. A spectral feature arising from a single neutral cytosine was identified at 1528 cm(-1). At low pH, this mode was replaced with...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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PMID: 17924627
DOI: 10.1021/ja0743893

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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