Article
Chinese hamster cell mutant, V-C8, a model for analysis of Brca2 function.
Mutation research - 30 Aug 2006
Wiegant Wouter W, Overmeer René M, Godthelp Barbara C, van Buul Paul P W, Zdzienicka Małgorzata Z
Abstract excerpt
The previously described Chinese hamster cell mutant V-C8 that is defective in Brca2 shows a very complex phenotype, including increased sensitivity towards a wide variety of DNA damaging agents, chromosomal instability, abnormal centrosomes and impaired formation of Rad51 foci in response to DNA damage. Here, we demonstrate that V-C8 cells display biallelic nonsense mutations in Brca2, one in exon 15 and the...
Topics
- Alleles
- Amino Acid Sequence
- Animals
- BRCA2 Protein
- Cell Line
- Centrosome
- Chromosomal Instability
- Chromosome Aberrations
- Codon, Nonsense
- Codon, Terminator
- Cricetinae
- Cricetulus
- Cross-Linking Reagents
- Female
- Genes, BRCA2
- Heterozygote
- Models, Genetic
- Molecular Sequence Data