Article
A new complementation group of mitomycin C-hypersensitive Chinese hamster cell mutants that closely resembles the phenotype of fanconi anemia cells.
Cancer research - 1 Aug 1995
Telleman P, Overkamp W J, van Wessel N, Studzian K, Wetselaar L, Natarajan A T, Zdzienicka M Z
Abstract excerpt
Three Mitomycin C (MMC)-hypersensitive mutants (CL-V1B, CL-V5B, and CL-V101B) were isolated from Chinese hamster V79B cells by the replica plating technique. In comparison to the parental cell line, CL-V1B, CL-V5B, and CL-V101B show about a 22-, 32-, and 13-fold increased sensitivity to MMC, respectively (judged by the D10). These mutants are also sensitive to other DNA cross-linking agents, such as...
Topics
- Alkylating Agents
- Animals
- CHO Cells
- Cell Survival
- Chromosome Aberrations
- Cricetinae
- Fanconi Anemia
- Genetic Complementation Test
- Hypoxanthine Phosphoribosyltransferase
- Mitomycin