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Article

Site-specific transformation of Drosophila via phiC31 integrase-mediated cassette exchange.

Genetics - 1 Jun 2006

Bateman Jack R, Lee Anne M, Wu C-ting

Abstract excerpt

Position effects can complicate transgene analyses. This is especially true when comparing transgenes that have inserted randomly into different genomic positions and are therefore subject to varying position effects. Here, we introduce a method for the precise targeting of transgenic constructs to predetermined genomic sites in Drosophila using the C31 integrase system in conjunction with recombinase-mediated...

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Identifiers

PMID: 16547094
DOI: 10.1534/genetics.106.056945
PMCID: PMC1526508

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