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Article

Exploiting position effects and the gypsy retrovirus insulator to engineer precisely expressed transgenes.

Nature genetics - 1 Apr 2008

Markstein Michele, Pitsouli Chrysoula, Villalta Christians, Celniker Susan E, Perrimon Norbert

Abstract excerpt

A major obstacle to creating precisely expressed transgenes lies in the epigenetic effects of the host chromatin that surrounds them. Here we present a strategy to overcome this problem, employing a Gal4-inducible luciferase assay to systematically quantify position effects of host chromatin and the ability of insulators to counteract these effects at phiC31 integration loci randomly distributed throughout the...

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PMID: 18311141
DOI: 10.1038/ng.101
PMCID: PMC2330261

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

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