P-element mutagenesis.

doi:10.1007/978-1-59745-583-1_6

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Article

P-element mutagenesis.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2008

Hummel Thomas, Klämbt Christian

Abstract excerpt

Mobile elements were first used as a mutagenesis tool that introduces a molecular tag in the genes of interest. This facilitated subsequent molecular cloning and eventually promoted molecular analysis of a large number of fly genes. Soon after, P-elements were modified to detect genes not only based on a mutant phenotype but rather through revealing RNA or protein expression patterns (enhancer trap, gene trap)....

Topics

Alternative Splicing
Animals
Crosses, Genetic
DNA Transposable Elements
Drosophila melanogaster
Enhancer Elements, Genetic
Female
Gene Expression Regulation
Genetic Techniques
Male
Models, Genetic
Mutagenesis
Mutagenesis, Site-Directed
Mutation
Recombination, Genetic

Source

Open in PubMed

Identifiers

PMID: 18641943
DOI: 10.1007/978-1-59745-583-1_6

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