Article
Fast and efficient Drosophila melanogaster gene knock-ins using MiMIC transposons.
G3 (Bethesda, Md.) - 8 Oct 2014
Vilain Sven, Vanhauwaert Roeland, Maes Ine, Schoovaerts Nils, Zhou Lujia, Soukup Sandra, da Cunha Raquel, Lauwers Elsa, Fiers Mark, Verstreken Patrik
Abstract excerpt
Modern molecular genetics studies necessitate the manipulation of genes in their endogenous locus, but most of the current methodologies require an inefficient donor-dependent homologous recombination step to locally modify the genome. Here we describe a methodology to efficiently generate Drosophila knock-in alleles by capitalizing on the availability of numerous genomic MiMIC transposon insertions carrying...
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