Article
A powerful method combining homologous recombination and site-specific recombination for targeted mutagenesis in Drosophila.
Proceedings of the National Academy of Sciences of the United States of America - 16 Sept 2008
Gao Guanjun, McMahon Conor, Chen Jie, Rong Yikang S
Abstract excerpt
Gene targeting provides a powerful tool for dissecting gene function. However, repeated targeting of a single locus remains a practice mostly limited to unicellular organisms that afford simple targeting methodologies. We developed an efficient method to repeatedly target a single locus in Drosophila. In this method, which we term "site-specific integrase mediated repeated targeting" (SIRT), an attP attachment...
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