Genome manipulations with bacterial recombineering and site-specific integration in Drosophila.

Abstract excerpt

Gene targeting is a vital tool for modern biology. The ability to efficiently and repeatedly target the same locus is made more efficient by the site-specific integrase mediated repeated targeting (SIRT) method, which combines homologous recombination, site-specific integration, and bacterial recombineering to conduct targeted modifications of individual loci. Here we describe the recombineering designs and...

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