Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Improving autofluorescent proteins: comparative studies of the effective brightness of Green Fluorescent Protein (GFP) mutants.

Microscopy research and technique - 1 Mar 2006

Jung Gregor, Zumbusch A

Abstract excerpt

We study the photophysical behavior of 8 mutants of Green Fluorescent Protein (GFP) using fluorescence correlation spectroscopy (FCS) on the single molecule level and double resonance excitation of bulk samples. Experimental data reported here and the previously published data on the RH/R(-) equilibrium and fluorescence quantum yields Phi(Fl) (Jung et al., 2005; Biophys J 88:1932-1947) are analyzed with respect...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Bacterial Proteins
Share to Electron Spin Resonance Spectroscopy

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Fluorescence Resonance Energy Transfer

Share to Green Fluorescent Proteins

Share to Luminescent Proteins

Share to Microscopy, Fluorescence

Share to Mutation

Share to Spectrometry, Fluorescence

Source

Identifiers

PMID: 16538631
DOI: 10.1002/jemt.20285

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Improving autofluorescent proteins: comparative studies of the effective brightness of Green Fluorescent Protein (GFP) mutants.PMID 16538631Read ↗

Efficient photoconversion distorts the fluorescence lifetime of GFP in confocal microscopy: a model kinetic study on mutant Thr203Val.Semantic proximity Read ↗

Autofluorescent proteins in single-molecule research: applications to live cell imaging microscopy.Semantic proximity Read ↗

Minimum set of mutations needed to optimize cyan fluorescent proteins for live cell imaging.Semantic proximity Read ↗

Diffusion-photodynamics coupling in Fluorescence Correlation Spectroscopy studies of photoswitchable Green Fluorescent Proteins: an analytical and simulative study.Semantic proximity Read ↗

The single T65S mutation generates brighter cyan fluorescent proteins with increased photostability and pH insensitivity.Semantic proximity Read ↗

Structural origin and rational development of bright red noncanonical variants of green fluorescent protein.Semantic proximity Read ↗

Bright GFP with subnanosecond fluorescence lifetime.Semantic proximity Read ↗

Mispacking and the Fitness Landscape of the Green Fluorescent Protein Chromophore Milieu.Semantic proximity Read ↗

[Fluorescent proteins: physical-chemical properties and application in cell biology].Semantic proximity Read ↗

YGFP: a spectral variant of GFP.Semantic proximity Read ↗

Complex fluorescence of the cyan fluorescent protein: comparisons with the H148D variant and consequences for quantitative cell imaging.Semantic proximity Read ↗

Brighter Red Fluorescent Proteins by Rational Design of Triple-Decker Motif.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.