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Article

The single T65S mutation generates brighter cyan fluorescent proteins with increased photostability and pH insensitivity.

PloS one - 1 Jan 2012

Fredj Asma, Pasquier Hélène, Demachy Isabelle, Jonasson Gabriella, Levy Bernard, Derrien Valérie, Bousmah Yasmina, Manoussaris Gallia, Wien Frank, Ridard Jacqueline, Erard Marie, Merola Fabienne

Abstract excerpt

Cyan fluorescent proteins (CFP) derived from Aequorea victoria GFP, carrying a tryptophan-based chromophore, are widely used as FRET donors in live cell fluorescence imaging experiments. Recently, several CFP variants with near-ultimate photophysical performances were obtained through a mix of site-directed and large scale random mutagenesis. To understand the structural bases of these improvements, we have...

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Identifiers

PMID: 23133673
DOI: 10.1371/journal.pone.0049149
PMCID: PMC3487735

Genes

CFP

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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