Article
Bright GFP with subnanosecond fluorescence lifetime.
Scientific reports - 5 Sept 2018
Mamontova Anastasia V, Solovyev Ilya D, Savitsky Alexander P, Shakhov Alexander М, Lukyanov Konstantin A, Bogdanov Alexey M
Abstract excerpt
Fluorescence lifetime imaging microscopy (FLIM) measures fluorescence decay rate at every pixel of an image. FLIM can separate probes of the same color but different fluorescence lifetimes (FL), thus it is a promising approach for multiparameter imaging. However, available GFP-like fluorescent proteins (FP) possess a narrow range of FLs (commonly, 2.3-3.5 ns) which limits their applicability for multiparameter...
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