Article
Structural origin and rational development of bright red noncanonical variants of green fluorescent protein.
Physical chemistry chemical physics : PCCP - 15 Jun 2023
Chen Cheng, Zhang Hao, Zhang Jing, Ai Hui-Wang, Fang Chong
Abstract excerpt
The incorporation of noncanonical amino acids (ncAAs) into fluorescent proteins is promising for red-shifting their fluorescence and benefiting tissue imaging with deep penetration and low phototoxicity. However, ncAA-based red fluorescent proteins (RFPs) have been rare. The 3-aminotyrosine modified superfolder green fluorescent protein (aY-sfGFP) represents a recent advance, yet the molecular mechanism for its...
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