Article
Diffusion-photodynamics coupling in Fluorescence Correlation Spectroscopy studies of photoswitchable Green Fluorescent Proteins: an analytical and simulative study.
The journal of physical chemistry. B - 1 Sept 2011
Daglio S C, Banterle N, D'Alfonso L, Collini M, Chirico G
Abstract excerpt
The photodynamics of the Green Fluorescent Protein (GFP) has been addressed in detail, particularly by means of Fluorescence Correlation Spectroscopy (FCS), a technique that provides direct information when the diffusion and the photodynamics time scales are well separated. Efficient photoswitchable GFPs, a crucial component for applications in nanoscopy imaging, have long residence times in the dark state,...
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