Article
Autofluorescent proteins in single-molecule research: applications to live cell imaging microscopy.
Biophysical journal - 1 May 2001
Harms G S, Cognet L, Lommerse P H, Blab G A, Schmidt T
Abstract excerpt
The spectral and photophysical characteristics of the autofluorescent proteins were analyzed and compared to flavinoids to test their applicability for single-molecule microscopy in live cells. We compare 1) the number of photons emitted by individual autofluorescent proteins in artificial and in vivo situations, 2) the saturation intensities of the various autofluorescent proteins, and 3) the maximal emitted...
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