Article
Complex fluorescence of the cyan fluorescent protein: comparisons with the H148D variant and consequences for quantitative cell imaging.
Biochemistry - 25 Nov 2008
Villoing Aude, Ridhoir Myriam, Cinquin Bertrand, Erard Marie, Alvarez Luis, Vallverdu Germain, Pernot Pascal, Grailhe Régis, Mérola Fabienne, Pasquier Hélène
Abstract excerpt
We have studied the fluorescence decays of the purified enhanced cyan fluorescent protein (ECFP, with chromophore sequence Thr-Trp-Gly) and of its variant carrying the single H148D mutation characteristic of the brighter form Cerulean. Both proteins exhibit highly complex fluorescence decays showing strong temperature and pH dependences. At neutral pH, the H148D mutation leads (i) to a general increase in all...
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