Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Mispacking and the Fitness Landscape of the Green Fluorescent Protein Chromophore Milieu.

Biochemistry - 7 Feb 2017

Banerjee Shounak, Schenkelberg Christian D, Jordan Thomas B, Reimertz Julia M, Crone Emily E, Crone Donna E, Bystroff Christopher

Abstract excerpt

The autocatalytic maturation of the chromophore in green fluorescent protein (GFP) was thought to require the precise positioning of the side chains surrounding it in the core of the protein, many of which are strongly conserved among homologous fluorescent proteins. In this study, we screened for green fluorescence in an exhaustive set of point mutations of seven residues that make up the chromophore...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Amino Acids
Share to Crystallography, X-Ray

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Fluorescence

Share to Green Fluorescent Proteins

Share to Mutagenesis, Site-Directed

Share to Recombinant Proteins

Share to Spectrometry, Fluorescence

Share to Structure-Activity Relationship

Source

Open in PubMed Open full text

Identifiers

PMID: 28074648
DOI: 10.1021/acs.biochem.6b00800
PMCID: PMC6193456

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 2 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Mispacking and the Fitness Landscape of the Green Fluorescent Protein Chromophore Milieu.PMID 28074648Read ↗

Heterogeneity of the GFP fitness landscape and data-driven protein design.Semantic proximity Read ↗

Functional tuning of a salvaged green fluorescent protein variant with a new sequence space by directed evolution.Semantic proximity Read ↗

Mechanism and energetics of green fluorescent protein chromophore synthesis revealed by trapped intermediate structures.Semantic proximity Read ↗

A hinge migration mechanism unlocks the evolution of green-to-red photoconversion in GFP-like proteins.Semantic proximity Read ↗

Turning up the Green Light.Semantic proximity Read ↗

The first mutant of the Aequorea victoria green fluorescent protein that forms a red chromophore.Semantic proximity Read ↗

Designed active-site library reveals thousands of functional GFP variants.Semantic proximity Read ↗

Local fitness landscape of the green fluorescent protein.Semantic proximity Read ↗

Crystal Structure of Green Fluorescent Protein Clover and Design of Clover-Based Redox Sensors.Semantic proximity Read ↗

Structure and mechanism of the photoactivatable green fluorescent protein.Semantic proximity Read ↗

Recovery of red fluorescent protein chromophore maturation deficiency through rational design.Semantic proximity Read ↗

Exploring the functional residues in a flavin-binding fluorescent protein using deep mutational scanning.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.