Article
Efficient photoconversion distorts the fluorescence lifetime of GFP in confocal microscopy: a model kinetic study on mutant Thr203Val.
Chemphyschem : a European journal of chemical physics and physical chemistry - 15 Sept 2008
Jung Gregor, Werner Michael, Schneider Marc
Abstract excerpt
Phototransformations of autofluorescent proteins are applied in high-resolution microscopy and in studying cellular transport, but they are detrimental when accidentally occurring in blinking or photobleaching (BL). Here, we investigate the kinetics of phototransformations of a photoactivatable green fluorescent protein (GFP) in confocal microscopy. Photoconversion (PC) is achieved by excitation of the barely...
Topics
- Fluorescence
- Green Fluorescent Proteins
- Kinetics
- Microscopy, Confocal
- Models, Biological
- Molecular Structure
- Mutation
- Photochemistry
- Threonine
- Time Factors
- Valine