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Article

Reverse genetics system for introduction of site-specific mutations into the double-stranded RNA genome of infectious rotavirus.

Proceedings of the National Academy of Sciences of the United States of America - 21 Mar 2006

Komoto Satoshi, Sasaki Jun, Taniguchi Koki

Abstract excerpt

We describe here the successful establishment of a reverse genetics system for rotavirus (RV), a member of the Reoviridae family whose genome consists of 10-12 segmented dsRNA. The system is based on the recombinant vaccinia virus T7 RNA polymerase-driven procedure for supplying artificial viral mRNA in the cytoplasm. With the aid of helper virus (human RV strain KU) infection, intracellularly transcribed...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Genome, Viral

Share to Mutagenesis, Site-Directed

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Source

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Identifiers

PMID: 16537420
DOI: 10.1073/pnas.0509385103
PMCID: PMC1450225

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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