Article
Dual selection mechanisms drive efficient single-gene reverse genetics for rotavirus.
Proceedings of the National Academy of Sciences of the United States of America - 26 Oct 2010
Trask Shane D, Taraporewala Zenobia F, Boehme Karl W, Dermody Terence S, Patton John T
Abstract excerpt
Current methods for engineering the segmented double-stranded RNA genome of rotavirus (RV) are limited by inefficient recovery of the recombinant virus. In an effort to expand the utility of RV reverse genetics, we developed a method to recover recombinant viruses in which independent selection strategies are used to engineer single-gene replacements. We coupled a mutant SA11 RV encoding a temperature-sensitive...
Topics
- Amino Acid Sequence
- Base Sequence
- Cell Line
- DNA, Viral
- Genes, Viral
- Genetic Engineering
- Humans
- Molecular Sequence Data
- Mutation
- RNA Interference
- RNA-Binding Proteins
- Recombinant Proteins
- Recombination, Genetic