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Article

Development of an entirely plasmid-based reverse genetics system for 12-segmented double-stranded RNA viruses.

Proceedings of the National Academy of Sciences of the United States of America - 19 Oct 2021

Nouda Ryotaro, Minami Shohei, Kanai Yuta, Kawagishi Takahiro, Nurdin Jeffery A, Yamasaki Moeko, Kuwata Ryusei, Shimoda Hiroshi, Maeda Ken, Kobayashi Takeshi

Abstract excerpt

The family Reoviridae is a nonenveloped virus group with a double-stranded (ds) RNA genome comprising 9 to 12 segments. In the family Reoviridae, the genera Cardoreovirus, Phytoreovirus, Seadornavirus, Mycoreovirus, and Coltivirus contain virus species having 12-segmented dsRNA genomes. Reverse genetics systems used to generate recombinant infectious viruses are powerful tools for investigating viral gene...

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Cricetinae
Genome, Viral
Glycosylation
Mutation
Plasmids

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Reassortant Viruses

Viral gastroenteritis research and epidemiology

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Identifiers

PMID: 34635593
DOI: 10.1073/pnas.2105334118
PMCID: PMC8594578

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