Article
Site-specific recombination of asymmetric lox sites mediated by a heterotetrameric Cre recombinase complex.
Bioorganic & medicinal chemistry - 1 May 2006
Saraf-Levy Talia, Santoro Stephen W, Volpin Hanne, Kushnirsky Tzvika, Eyal Yoram, Schultz Peter G, Gidoni David, Carmi Nir
Abstract excerpt
Previous reports have demonstrated that new Cre recombinase specificities can be developed for symmetrically designed lox mutants through directed evolution. The development of Cre variants that allow the recombination of true asymmetric lox mutant sites has not yet been addressed, however. In the present study, we demonstrate that a mixture of two different site-specific Cre recombinase molecules (wt Cre and a...
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