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Article

A genetic screen identifies novel non-compatible loxP sites.

Nucleic acids research - 15 Jul 2002

Langer Stephen J, Ghafoori A Paiman, Byrd Marshall, Leinwand Leslie

Abstract excerpt

The ability of the Cre/lox system to make precise genomic modifications is a tremendous accomplishment. However, recombination between cis-linked heterospecific lox sites limits the use of Cre- mediated exchange of DNA to systems where genetic selection can be applied. To circumvent this problem we carried out a genetic screen designed to identify novel mutant spacer-containing lox sites displaying enhanced...

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Identifiers

PMID: 12136089
DOI: 10.1093/nar/gkf421
PMCID: PMC135742

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