Functional mapping of Cre recombinase by pentapeptide insertional mutagenesis.

Abstract excerpt

Cre is a site-specific recombinase from bacteriophage P1. It is a member of the tyrosine integrase family and catalyzes reciprocal recombination between specific 34-bp sites called loxP. To analyze the structure-function relationships of this enzyme, we performed large scale pentapeptide insertional mutagenesis to generate insertions of five amino acids at random positions in the protein. The high density of...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Catalysis
• Share to Gene Library
• Share to Integrases