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Article

Engineering spacer specificity of the Cre/loxP system.

Nucleic acids research - 22 Jul 2024

Hoersten Jenna, Ruiz-Gómez Gloria, Paszkowski-Rogacz Maciej, Gilioli Giorgio, Guillem-Gloria Pedro Manuel, Lansing Felix, Pisabarro M Teresa, Buchholz Frank

Abstract excerpt

Translational research on the Cre/loxP recombination system focuses on enhancing its specificity by modifying Cre/DNA interactions. Despite extensive efforts, the exact mechanisms governing Cre discrimination between substrates remains elusive. Cre recognizes 13 bp inverted repeats, initiating recombination in the 8 bp spacer region. While literature suggests that efficient recombination proceeds between lox...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Identifiers

PMID: 38869070
DOI: 10.1093/nar/gkae481
PMCID: PMC11260471

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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