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Article

In vivo random mutagenesis of Bacillus subtilis by use of TnYLB-1, a mariner-based transposon.

Applied and environmental microbiology - 1 Jan 2006

Le Breton Yoann, Mohapatra Nrusingh Prasad, Haldenwang W G

Abstract excerpt

This report describes the construction and characterization of a mariner-based transposon system designed to be used in Bacillus subtilis, but potentially applicable to other gram-positive bacteria. Two pUC19-derived plasmids were created that contain the mariner-Himar1 transposase gene, modified for expression in B. subtilis, under the control of either sigmaA- or sigmaB-dependent promoters. Both plasmids also...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Identifiers

PMID: 16391061
DOI: 10.1128/AEM.72.1.327-333.2006
PMCID: PMC1352254

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Source receipt: pubmed, retrieved 7 Sept 2026.

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