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Random mutagenesis of the hyperthermophilic archaeon Pyrococcus furiosus using in vitro mariner transposition and natural transformation.

Scientific reports - 8 Nov 2016

Guschinskaya Natalia, Brunel Romain, Tourte Maxime, Lipscomb Gina L, Adams Michael W W, Oger Philippe, Charpentier Xavier

Abstract excerpt

Transposition mutagenesis is a powerful tool to identify the function of genes, reveal essential genes and generally to unravel the genetic basis of living organisms. However, transposon-mediated mutagenesis has only been successfully applied to a limited number of archaeal species and has never been reported in Thermococcales. Here, we report random insertion mutagenesis in the hyperthermophilic archaeon...

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Identifiers

PMID: 27824140
DOI: 10.1038/srep36711
PMCID: PMC5099854

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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