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Article

Generation of a Transposon Mutant Library in Staphylococcus aureus and Staphylococcus epidermidis Using bursa aurealis.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2016

Yajjala Vijaya Kumar, Widhelm Todd J, Endres Jennifer L, Fey Paul D, Bayles Kenneth W

Abstract excerpt

Transposon mutagenesis is a genetic process that involves the random insertion of transposons into a genome resulting in the disruption of function of the genes in which they insert. Identification of the insertion sites through DNA sequencing allows for the identification of the genes disrupted and the creation of "libraries" containing a collection of mutants in which a large number of the nonessential genes...

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PMID: 25682373
DOI: 10.1007/7651_2014_189

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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