Article
Transposon-mediated random mutagenesis of Bacillus subtilis.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2011
Wilson Adam C, Szurmant Hendrik
Abstract excerpt
The depth of knowledge concerning its physiology and genetics make Bacillus subtilis an attractive system for strain engineering and analysis. Transposon-based mutagenesis strategies generate large libraries of mutant strains that can be used to investigate molecular mechanisms relevant in fundamental research or to generate desirable phenotypes in applied research. This section presents a mini-Tn10-based...
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