Implementation of a loss-of-function system to determine growth and stress-associated mutagenesis in Bacillus subtilis.

doi:10.1371/journal.pone.0179625

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Article

Implementation of a loss-of-function system to determine growth and stress-associated mutagenesis in Bacillus subtilis.

PloS one - 1 Jan 2017

Villegas-Negrete Norberto, Robleto Eduardo A, Obregón-Herrera Armando, Yasbin Ronald E, Pedraza-Reyes Mario

Abstract excerpt

A forward mutagenesis system based on the acquisition of mutations that inactivate the thymidylate synthase gene (TMS) and confer a trimethoprim resistant (Tmpr) phenotype was developed and utilized to study transcription-mediated mutagenesis (TMM). In addition to thyA, Bacillus subtilis possesses thyB, whose expression occurs under conditions of cell stress; therefore, we generated a thyB- thyA+ mutant strain....

Topics

Bacillus subtilis
Bacterial Proteins
Mutagenesis
Mutation
Thymidylate Synthase
Trimethoprim

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Identifiers

PMID: 28700593
DOI: 10.1371/journal.pone.0179625
PMCID: PMC5507404

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