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Article

Reversion of recombinant toxoids: mutations in diphtheria toxin that partially compensate for active-site deletions.

Proceedings of the National Academy of Sciences of the United States of America - 1 Jul 1992

Killeen K P, Escuyer V, Mekalanos J J, Collier R J

Abstract excerpt

Deleting an important active-site residue of diphtheria toxin, glutamic acid-148, reduces the toxin's ADP-ribosyltransferase activity by a factor of greater than 10(4). We considered using this mutation to construct a recombinant toxoid for expression by live attenuated vaccines and explored second-site mutations that might cause reversion. Activity was partially restored by substituting glutamic acid for...

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Identifiers

PMID: 1631110
DOI: 10.1073/pnas.89.13.6207
PMCID: PMC402151

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