Active-site mutations of diphtheria toxin: role of tyrosine-65 in NAD binding and ADP-ribosylation.

doi:10.1021/bi00255a031

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Article

Active-site mutations of diphtheria toxin: role of tyrosine-65 in NAD binding and ADP-ribosylation.

Biochemistry - 27 Dec 1994

Blanke S R, Huang K, Collier R J

Abstract excerpt

Previous studies have suggested that tyrosine-65 (Tyr-65) of diphtheria toxin (DT) is located at the active site. To investigate the role of Tyr-65 in NAD binding and the ADP-ribosylation of elongation factor-2 (EF-2), we changed this residue to alanine and phenylalanine by site-directed mutagene...

Topics

Adenosine
Binding Sites
Diphtheria Toxin
In Vitro Techniques
Mutagenesis, Site-Directed
Mutation
NAD
Poly(ADP-ribose) Polymerases
Recombinant Proteins
Spectrometry, Fluorescence
Structure-Activity Relationship
Thermodynamics

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