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Article

Active-site mutations of diphtheria toxin: role of tyrosine-65 in NAD binding and ADP-ribosylation.

Biochemistry - 27 Dec 1994

Blanke S R, Huang K, Collier R J

Abstract excerpt

Previous studies have suggested that tyrosine-65 (Tyr-65) of diphtheria toxin (DT) is located at the active site. To investigate the role of Tyr-65 in NAD binding and the ADP-ribosylation of elongation factor-2 (EF-2), we changed this residue to alanine and phenylalanine by site-directed mutagenesis of a synthetic gene encoding the catalytic fragment of DT (DTA). The alanine mutant was greatly diminished in...

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Identifiers

PMID: 7803411
DOI: 10.1021/bi00255a031

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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