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Article

Construction and expression of plasmids containing mutated diphtheria toxin A-chain-coding sequences.

Infection and immunity - 1 Oct 1991

Fisher K S, Maxwell I H, Murphy J R, Collier J, Glode L M

Abstract excerpt

We previously demonstrated that cells can be killed through transfection of an expression plasmid that encodes the diphtheria toxin A-chain fragment (DT-A). This report describes the construction of expression plasmids containing three mutant DT-A-coding sequences substituting glutamic acid 148 with aspartic acid, serine, or glutamine which are known to have 100- to 300-fold-reduced ADP-ribosylation activity...

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Identifiers

PMID: 1894362
DOI: 10.1128/iai.59.10.3562-3565.1991
PMCID: PMC258921

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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