Construction and expression of plasmids containing mutated diphtheria toxin A-chain-coding sequences.

doi:10.1128/iai.59.10.3562-3565.1991

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Article

Construction and expression of plasmids containing mutated diphtheria toxin A-chain-coding sequences.

Infection and immunity - 1 Oct 1991

Fisher K S, Maxwell I H, Murphy J R, Collier J, Glode L M

Abstract excerpt

We previously demonstrated that cells can be killed through transfection of an expression plasmid that encodes the diphtheria toxin A-chain fragment (DT-A). This report describes the construction of expression plasmids containing three mutant DT-A-coding sequences substituting glutamic acid 148 w...

Topics

Diphtheria Toxin
Gene Expression
HeLa Cells
Humans
Mutation
Peptide Fragments
Plasmids
Transfection

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