Selection of diphtheria toxin active-site mutants in yeast. Rediscovery of glutamic acid-148 as a key residue.

doi:10.1007/978-1-4419-8632-0_5

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Article

Selection of diphtheria toxin active-site mutants in yeast. Rediscovery of glutamic acid-148 as a key residue.

Advances in experimental medicine and biology - 1 Jan 1997

Fu H, Blanke S R, Mattheakis L C, Collier R J

Abstract excerpt

Saccharomyces cerevisiae was transformed with expression plasmids carrying the DTA gene under control of the GAL1 promoter; colonies that formed under inducing conditions were selected; and plasmids from these colonies were screened for mutations in DTA that failed to block expression of the prot...

Topics

Binding Sites
Diphtheria Toxin
Enzyme Inhibitors
Genetic Vectors
Glutamic Acid
Molecular Structure
Mutation
Poly(ADP-ribose) Polymerase Inhibitors
Recombinant Fusion Proteins
Saccharomyces cerevisiae

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