Article
Engineering of genetically detoxified pertussis toxin analogs for development of a recombinant whooping cough vaccine.
Infection and immunity - 1 Nov 1990
Loosmore S M, Zealey G R, Boux H A, Cockle S A, Radika K, Fahim R E, Zobrist G J, Yacoob R K, Chong P C, Yao F L
Abstract excerpt
Pertussis toxin (PT) is an important protective antigen in vaccines against whooping cough, and a genetically detoxified PT analog is the preferred form of the immunogen. Several amino acids of the S1 subunit were identified as functionally critical residues by site-directed mutagenesis, specific...
Topics
- Amino Acid Sequence
- Animals
- Cell Line
- Cloning, Molecular
- Cricetinae
- Cricetulus
- Female
- Gene Expression
- Mice
- Mice, Inbred Strains
- Molecular Sequence Data
- Mutation
- Operon
- Pertussis Toxin
- Pertussis Vaccine