Article
Construction of nontoxic derivatives of cholera toxin and characterization of the immunological response against the A subunit.
Infection and immunity - 1 Jun 1995
Fontana M R, Manetti R, Giannelli V, Magagnoli C, Marchini A, Olivieri R, Domenighini M, Rappuoli R, Pizza M
Abstract excerpt
Using computer modelling, we have identified some of the residues of the A subunit of cholera toxin (CT) and heat-labile toxin that are involved in NAD binding, catalysis, and toxicity. Here we describe the site-directed mutagenesis of the CT gene and the construction of CT mutants. Nine mutations of the A subunit gene were generated. Six of them encoded proteins that were fully assembled in the AB5 structure and...
Topics
- Animals
- Base Sequence
- Cholera Toxin
- Cholera Vaccines
- Immunization
- Molecular Sequence Data
- Mutation
- Rabbits
- Structure-Activity Relationship