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Article

Construction of nontoxic derivatives of cholera toxin and characterization of the immunological response against the A subunit.

Infection and immunity - 1 Jun 1995

Fontana M R, Manetti R, Giannelli V, Magagnoli C, Marchini A, Olivieri R, Domenighini M, Rappuoli R, Pizza M

Abstract excerpt

Using computer modelling, we have identified some of the residues of the A subunit of cholera toxin (CT) and heat-labile toxin that are involved in NAD binding, catalysis, and toxicity. Here we describe the site-directed mutagenesis of the CT gene and the construction of CT mutants. Nine mutations of the A subunit gene were generated. Six of them encoded proteins that were fully assembled in the AB5 structure and...

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Identifiers

PMID: 7768621
DOI: 10.1128/iai.63.6.2356-2360.1995
PMCID: PMC173311

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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