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Article

Gene replacement in Bordetella pertussis by transformation with linear DNA.

Bio/technology (Nature Publishing Company) - 1 Nov 1990

Zealey G R, Loosmore S M, Yacoob R K, Cockle S A, Boux L J, Miller L D, Klein M H

Abstract excerpt

We replaced the wild-type TOX operon of Bordetella pertussis with in vitro mutated, detoxified alleles by electroporetic transformation using unmarked linear DNA. Uptake of DNA was selected by transient ampicillin resistance and two simultaneous recombination events resulted in gene-replacement at the natural locus with no integration of heterologous DNA. TOX alleles were stable without selection and recombinant...

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Share to Alleles
Share to DNA, Bacterial
Share to Electrophoresis, Polyacrylamide Gel

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Source

Identifiers

PMID: 1366929
DOI: 10.1038/nbt1190-1025

Genes

TOX

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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