Gene replacement in Bordetella pertussis by transformation with linear DNA.

doi:10.1038/nbt1190-1025

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Article

Gene replacement in Bordetella pertussis by transformation with linear DNA.

Bio/technology (Nature Publishing Company) - 1 Nov 1990

Zealey G R, Loosmore S M, Yacoob R K, Cockle S A, Boux L J, Miller L D, Klein M H

Abstract excerpt

We replaced the wild-type TOX operon of Bordetella pertussis with in vitro mutated, detoxified alleles by electroporetic transformation using unmarked linear DNA. Uptake of DNA was selected by transient ampicillin resistance and two simultaneous recombination events resulted in gene-replacement a...

Topics

Alleles
Animals
Bordetella pertussis
DNA, Bacterial
Electrophoresis, Polyacrylamide Gel
Mice
Mutation
Operon
Pertussis Toxin
Recombinant Fusion Proteins
Transformation, Bacterial
Vaccines, Synthetic
Virulence Factors, Bordetella
Whooping Cough

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Open in PubMed

Identifiers

PMID: 1366929
DOI: 10.1038/nbt1190-1025

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Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-1366929-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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