Article
Pseudomonas aeruginosa exotoxin A: alterations of biological and biochemical properties resulting from mutation of glutamic acid 553 to aspartic acid.
Biochemistry - 29 May 1990
Douglas C M, Collier R J
Abstract excerpt
Glutamic acid 553 of Pseudomonas aeruginosa exotoxin A (ETA) was identified earlier as a putative active-site residue by photoaffinity labeling with NAD. Here ETA-E553D, a cloned form of the toxin in which Glu-553 has been replaced by aspartic acid, was purified from Escherichia coli extracts and...
Topics
- ADP Ribose Transferases
- Affinity Labels
- Animals
- Aspartic Acid
- Bacterial Toxins
- Clone Cells
- Electrophoresis, Polyacrylamide Gel
- Escherichia coli
- Exotoxins
- Genes, Bacterial
- Glutamates
- Glutamic Acid
- Kinetics
- Mutation
- Poly(ADP-ribose) Polymerases
- Pseudomonas aeruginosa
- Rats
- Thermolysin