Article
Efficient gene-driven germ-line point mutagenesis of C57BL/6J mice.
BMC genomics - 21 Nov 2005
Michaud Edward J, Culiat Cymbeline T, Klebig Mitchell L, Barker Paul E, Cain K T, Carpenter Debra J, Easter Lori L, Foster Carmen M, Gardner Alysyn W, Guo Z Y, Houser Kay J, Hughes Lori A, Kerley Marilyn K, Liu Zhaowei, Olszewski Robert E, Pinn Irina, Shaw Ginger D, Shinpock Sarah G, Wymore Ann M, Rinchik Eugene M, Johnson Dabney K
Abstract excerpt
BACKGROUND: Analysis of an allelic series of point mutations in a gene, generated by N-ethyl-N-nitrosourea (ENU) mutagenesis, is a valuable method for discovering the full scope of its biological function. Here we present an efficient gene-driven approach for identifying ENU-induced point mutations in any gene in C57BL/6J mice. The advantage of such an approach is that it allows one to select any gene of interest...
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