Article
A gene-driven ENU-based approach to generating an allelic series in any gene.
Mammalian genome : official journal of the International Mammalian Genome Society - 1 Aug 2004
Quwailid Mohamed Mohideen, Hugill Alison, Dear Neil, Vizor Lucie, Wells Sara, Horner Emma, Fuller Shelly, Weedon Jessica, McMath Hamish, Woodman Paul, Edwards David, Campbell David, Rodger Susan, Carey Joanne, Roberts Ann, Glenister Pete, Lalanne Zuzanna, Parkinson Nick, Coghill Emma L, McKeone Richard, Cox Sam, Willan John, Greenfield Andy, Keays David, Brady Saffron, Spurr Nigel, Gray Ian, Hunter Jackie, Brown Steve D M, Cox Roger D
Abstract excerpt
N-ethyl-N-nitrosourea (ENU) introduces mutations throughout the mouse genome at relatively high efficiency. Successful high-throughput phenotype screens have been reported and alternative screens using sequence-based approaches have been proposed. For the purpose of generating an allelic series in selected genes by a sequence-based approach, we have constructed an archive of over 4000 DNA samples from individual...
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