Article
Insights into the quality of DnaA boxes and their cooperativity.
Journal of molecular biology - 6 Jan 2006
Hansen Flemming G, Christensen Bjarke Bak, Nielsen Christina Bang, Atlung Tove
Abstract excerpt
Plasmids carrying the mioC promoter region with its two DnaA boxes are as efficient in titration of DnaA protein as plasmids carrying a replication-inactivated oriC region with its five DnaA boxes. The two DnaA boxes upstream of the mioC promoter were mutated in various ways to study the cooperativity between the DnaA boxes, and to study in vivo the in vitro-defined 9mer DnaA box consensus sequence...
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